Figure 6 | Scientific Reports

Figure 6

From: Quantification of the morphological characteristics of hESC colonies

Figure 6

(a) The number of cells \({N}_{c}\) as a function of the colony area \(A\) (log-log scale). Data points are coloured according to the number of days after plating in which the image was taken. The red dotted line corresponds to the best fit to \({N}_{C}(A)=\kappa {A}^{\beta }\), with a scaling factor \(\kappa =2130\) and exponent \(\beta =0.93\) (\({R}^{2}\) = 0.97). The three outliers for \(A < 0.01{\text{mm}}^{2}\) correspond to colonies with distinctive features when compared to the rest (partly differentiated). (b) Mean area per single cell, \(\bar{A}=A/{N}_{c}\) are the following \(\langle A/{N}_{c}\rangle =433\pm 57\ \mu \)m\({}^{2}\) (day 2), \(434\pm 109\ \mu \)m\({}^{2}\) (day 3) and \(564\pm 135\ \mu \)m\({}^{2}\) (day 4). The medians are shown as red central lines on each box, which edges represent the 25th and 75th percentiles respectively. Some data points at day 3 (crossed on top with +) were considered outliers. The points that correspond to colonies with differentiated cells are filled in black.

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