Table 3 Artefacts with attached plant and animal residues identified from the presence of plant- and animal-derived analytes detected by GC-MS/MS.

From: Combined organic biomarker and use-wear analyses of stone artefacts from Liang Bua, Flores, Indonesia

Artefact

Long-chain saturated fatty acids and analogues

Sterols

Terpenoids

Terpenes

XXIV-68

Tricosanoic (29 ng) and tetracosanoic (165 ng) acids

Cholesterol (15 ng) and β-sitosterol (detectablec)

Abietic (3 ng) and ursolic (detectablec) acids

—

XXIV-67a,b

Cholesterol (17 ng), oleanolic acid (350 ng) and ursolic acid (28 ng) were observed, as well as betulinic acid at detectable levels. Azelaic (detectablec), sebacic (134 ng) and thapsic (138 ng) acids were found, as were 12-hydroxydodecanoic (detectablec) and 16-hydroxyhexadecanoic (detectablec) acids. A suite of saturated fatty acids from pentadecanoic acid (56 ng) to tetracosanoic acid (34 ng) was also detected.

XXV-3932

Tetracosanoic (626 ng) and azelaic (detectablec) acids

Cholesterol (45 ng) and β-sitosterol (detectablec)

—

—

XXV-3955

Tetracosanoic acid (99 ng)

Cholesterol (207 ng) and β-sitosterol (detectablec)

—

—

XXV-3953

Azelaic acid (detectablec)

Cholesterol (63 ng), campesterol and β-sitosterol (detectablec)

—

Camphor (46 ng)

XXV-3954b

Cholesterol (100 ng), abietic acid (37 ng), β-sitosterol (detectablec), tridecanoic (detectablec), nonadecanoic (detectablec), eicosanoic (556 ng), docosanoic (detectablec) and azelaic (detectablec) acids were found. Camphor (43 ng) was extracted from the artefact.

XXV-4025b

Cholesterol (1.4 µg) was extracted from this artefact. β-Sitosterol (detectablec), tridecanoic (detectablec) and heptadecanoic (detectablec) acids were also detected.

XXV-4619

Tetracosanoic acid (1 µg)

Cholesterol (219 ng) and β-sitosterol (detectablec)

—

—

XXVI-4414b

Cholesterol (32 ng), β-sitosterol (detectablec) and stigmasterol (detectablec) were detected, as well as heptadecanoic (detectablec), nonadecanoic (detectablec) and eicosanoic (545 ng) acids.

XXVI-4532

—

Cholesterol (553 ng)

Oleanolic acid (890 ng)

—

  1. aIntepreted as unused after use-wear analysis.
  2. bGC-MS/MS data obtained from previous studies7,8.
  3. cAnalyte concentration in the extract was between the limits of detection and quantification.