Table 1 Summary of expression of targets in total protein of E. coli as determined by anti-His Western. Abbreviations: −, not expressed; +, expressed; NoC, no colonies obtained upon transforming the strain; nd, not done.
Protein | Strain | ||||
|---|---|---|---|---|---|
C43 (DE3) | BL21 (DE3) | KTD101 (DE3) | BL21-AI | Lemo21 (DE3) | |
BBA57-His12 | − | + | + | + | − |
RevA-His12 | − | NoC | NoC | − | nd |
P13-His12 | − | − | − | − | − |
BbHtrA-His12 | − | NoC | − | + | nd |
Lmp1-His12 | Could not make this clone | ||||
BB0238-His12 | − | NoC | NoC | + | nd |
BB0323-His12 | − | NoC | NoC | + | NoC |
BB0405-His12 | − | − | − | − | − |
BB0406-His12 | Could not make this clone | ||||
DipA-His12 | − | − | NoC | + | − |
P66-His12 | NoC | NoC | NoC | NoC | NoC |
PelB-BB0238 | nd | nd | nd | + | nd |
PelB-P66 | nd | nd | nd | + | nd |
