Figure 2
From: A Simulated Microgravity Environment Causes a Sustained Defect in Epithelial Barrier Function
Low shear simulated microgravity exposure affects epithelial barrier formation. A) Model of polarized intestinal epithelial monolayers cultured on a semi-permeable support. (B) TER values (days 2–14) of HT-29.cl19a cells grown on semi-permeable inserts following culture under normal cell culture conditions (flask control), cultured on microcarrier beads alone (static control), or cultured on microcarrier beads and exposed to simulated microgravity (RWV). No statistically significant underlying differences in overall TER raw values were observed between conditions. (C) Data are shown as the % change in TER of cells exposed to microgravity vs. static (black) or flask controls (white). No statistically significant difference was observed in % change between the different culture conditions, although the % change in TER of cells from RWV relative to the static control was consistently lower (n = 4).
