Figure 4
From: A Simulated Microgravity Environment Causes a Sustained Defect in Epithelial Barrier Function
Simulated microgravity increases susceptibility of IECs to acetaldehyde-induced barrier dysfunction. (A) IECs grown on inserts for 11 days post-RWV culture exhibit a significant decrease in TER after 5hrs of acetaldehyde treatment (###p < 0.001 vs. RWV untreated) vs. static (***p < 0.001) and flask controls (***p < 0.001; n = 7). (B) IECs at 11-day post-RWV also showed an increase in FD4 permeability after acetaldehyde treatment vs. static and flask controls (n = 7). Similarly, panel C shows IECs at 14-day post-RWV exhibited a significant decrease in TER (*p < 0.05, n = 6) vs. untreated controls, and a non-significant decrease in TER vs. static and flask controls (n = 6). (D) IECs at 14-day post-RWV have increased permeability to FD4 in response to acetaldehyde treatment (###p < 0.001 vs. RWV untreated) vs. static and flask controls (***p < 0.001, n = 7).
