Figure 6
From: A Simulated Microgravity Environment Causes a Sustained Defect in Epithelial Barrier Function
Tight junction protein expression levels are not reduced by simulated microgravity conditions. HT-29.cl19a IECs cultured in tissue culture flasks (‘Flask’), the Biowiggler or the rotating wall vessel (RWV), were grown on transwells as monolayers and subsequently subjected to 0.5% acetaldehyde vapor for 5 hours. IEC monolayers were then lysed, processed for Western blotting and probed for the proteins indicated. (A) Representative blots probed for ZO-1, occludin and β-actin. (B) Quantification of densitometric analysis from Western blot experiments performed in duplicate (n  =  3–4 independent experiments). Non-contiguous sections of the same blot are indicated by a black line.
