Figure 2
From: β-Cyclodextrin counteracts obesity in Western diet-fed mice but elicits a nephrotoxic effect
2HP-β-CD prevents cholesterol and triglyceride accumulation in liver tissue. Assessment of the effect of the WD and 2HP-β-CD on hepatic lipid accumulation. (a) Free cholesterol visualized in liver tissue by means of the Filipin (blue) staining, accompanied by a nuclear staining (red). (b–d) Triglycerides, Phospholipids and NEFA content quantified in liver homogenate. (e) Lipid droplets (black arrow) visualized in liver tissue with the anti-PLIN-2 antibody. (f)Neutral lipids visualized in liver tissue with the ORO (red) histochemical stain. (g) Plasma ALAT levels. TGs = Triglycerides; PLs = Phospholipids; NEFA = Non-esterified free fatty acids; PLIN-2 = Perilipin-2; ORO = Oil Red O; ALAT = Alanine aminotransferase. Data shown as mean ± SEM. Statistical analysis of TGs, PLs, NEFA and plasma ALAT measurements was performed using the One-way ANOVA test with Bonferroni post-hoc analysis. *p > 0.05.
