Table 1 Comparison of RNA-seq methods to study biofluids and extracellular vesicles.
From: Performance assessment of total RNA sequencing of human biofluids and extracellular vesicles
reference | method | library prep | sample types | strandedness | contaminants | biotypes | ||||
|---|---|---|---|---|---|---|---|---|---|---|
small RNA | total RNA | mRNA | lncRNA | microRNA | circRNA | |||||
Li et al., 2014 | Ion Total RNA-seq v2 (Thermofisher) | + | − | EVs from serum and urine | stranded | 2–50% rRNA | + | − | + | − |
Qin et al., 2015 | TGIRT-seq | − | + | plasma (1 mL) | stranded | 0.9–6.3% rRNA | + | + | + | − |
Savelyeva et al., 2017 | SOLiD sequencing technology | − | + | blood, plasma, EVs from plasma | NA | 4–33% rRNA | + | + | + | − |
Amorim et al., 2017 | Ion Total RNA-seq v2 (Thermofisher) | + | − | EVs from plasma | stranded | NA | + | + | + | − |
Giraldez et al., 2019 Akat et al., 2019 | phospho-RNA-seq | + | − | plasma, bone marrow | NA | high YRNA and rRNA content (percentage not reported) | + | + | + | − |
Zhou et al., 2019 | SILVER-seq | − | + | serum (droplet) |  | NA | + | + | + | − |
this study | SMARTer-seq | − | + | plasma, urine, conditioned medium (200 µl) and EVs | stranded | mitochondrial rRNA depending on sample type | + | + | − | + |
