Figure 3
From: Lupus-like Disease in FcγRIIB−/− Mice Induces Osteopenia
FcγRIIB deletion increases osteoclast, but not osteoblast differentiation. (A) ALP (left) and mineralized bone nodule (right) in osteoblasts derived from long bones of 6- and 10-month-old FcγRIIB−/− males and WT controls. ALP activity (unit/mg) and mineralization or alizarin red concentration (mM) were quantified. (B) FcγRIIB−/− and WT osteoclasts were generated on glass coverslips in the presence of M-CSF and RANKL (left). TRAP-positive spreading osteoclasts containing more than 5 nuclei per area were quantified by OsteoMeasure software, (OsteoMetrics, right). Results are mean ± SEM. *p < 0.05 versus corresponding WT controls. ALP; alkaline phosphatase, N.Oc; osteoclast number and Ar; area. Scale bar: 100 μm.
