Table 1 Analysis of the changes in spatial distribution of AGP epitopes, detected in fruit after fungal infection (inoculation day and 16th day of fungal inoculation).

From: Immunocytochemical studies on the distribution of arabinogalactan proteins (AGPs) as a response to fungal infection in Malus x domestica fruit

Experiment I

Tissue level

Cellular level

Epidermal layer

Hypodermal layer

Parenchyma

Cell wall - plasma membrane

Cytoplasm compartments

mAb

Treatment

Treatment

C

FI

C

FI

C

FI

C

FI

C

FI

Inoculation day in

JIM13

+

+

+

+

+

+

+

+

LM2

+

−/+

+

−/+

−/+

−/+

+

+

LM14

+

+

+

+

+

+

+

+

MAC207

+

+

−/+

+

−/+

+

16 days 16

JIM13

+

++

+

++

+

++

+

++

++

LM2

−/+

+

−/+

−/+

−/+

−/+

−/+

+

+

LM14

+

++

+

++

+

++

+

++

+

MAC207

+

+

−/+

+

+

−/+

+

+

  1. Immunolabelling was evaluated according to Xie and co-workers (5): (−) no labelling, epitope not detected; (+) middle labelling; (++) very strong fluorescence signal. Abbreviations: C – control sample; FI – fruit after fungal infection
  2. The addition of the β-GlcY caused progression of the infection and thus destruction of the apple tissue, which resulted in an unclear pattern of AGP labelling with all the antibodies used. Analysis at the tissue level showed disorderly changes in AGP localization after 16 days of the experiment over the entire fruit surface. The progress of the fungal disease was correlated with displacement of AGP and disturbance in their uninterrupted occurrence in the cell wall-plasma membrane continuum.