Figure 2
From: A SUMO-dependent pathway controls elongating RNA Polymerase II upon UV-induced damage
Rpb1 of elongating RNAPII is SUMOylated upon UV treatment. (A) Rpb1-S2P and the corresponding SUMO-modified form in WT cells and cells expressing GFP-tagged SUMO after UV irradiation (400 J/m2) followed by a recovery time course in YPD medium. SUMOylation is indicated by mobility shift of Rpb1-S2P with the tagged SUMO protein. The 3E10 antibody was used to detect Rpb1. Dpm1 served as loading control. (B) Immunoprecipitation of Rpb1 with Rpb1-S2P-specific antibody (3E10) from UV-treated and untreated WT, ubc9-1, siz1Δ, siz2Δ and siz1Δ siz2Δ double mutant cells. SUMOylated species of Rpb1 were detected by western blotting (WB) using SUMO-specific antibody.
