Figure 4 | Scientific Reports

Figure 4

From: Simultaneous measurement of p53:Mdm2 and p53:Mdm4 protein-protein interactions in whole cells using fluorescence labelled foci

Figure 4

Titrations of the Mdm2 specific small molecule inhibitors Nutlin 3A and MI-773, and the small molecule Mdm2/Mdm4 dual inhibitor RO-5963, against the dual Mdm2:p53 and Mdm4:p53 FLUOPPI system at (A) 1 hour and (B) 24 hour time-points. IC50s were determined for each compound against each PPI (Mdm2:p53 and Mdm4:p53) using a 4 parameter model. Derived values are colour coded and labelled next to their respective fits. Curve fitting was performed using Prism 8 (Graphpad). Experiments were performed in DMEM cell media containing 10% (v/v) FCS.

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