Figure 3 | Scientific Reports

Figure 3

From: Minimally Invasive Delivery of Microbeads with Encapsulated, Viable and Quiescent Neural Stem Cells to the Adult Subventricular Zone

Figure 3

EC MMP Activity Facilitates Degradation of Microbeads. (A) Degradable microbeads in PBS, collagenase, NSC culture media (CM), NSC + EC CM, and EC CM, at 0, 1, and 2 days. Arrows point to areas of microbead surface corruption. (B) In situ zymography with no cells (control), NSC, NSC and EC and EC monolayers. Cells stained with Hoechst (top row) are incorporated into an agarose gel with fluorescein gelatin to detect cell-secreted MMPs (middle row), quantified in (C) using ImageJ (n = 3). Error bars represent SEM. Scale bar (50 µm) representative of all images. **p ≤ 0.01 determined by an unpaired t test.

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