Table 2 Cycling conditions for PST-PCR.

From: Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking

Reaction type

Number of cycles

Thermal conditions

First round

  

95 °C (2 min)

Linear

7–18

95 °C (15 sec), 65–72 °C (20 sec), 72 °C (1 min)

Exponential

12–18

95 °C (15 sec), 50–60 °C (15 sec), 72 °C (1 min)

Dilution 5–10 times

Second round

  

95 °C (1 min)

Exponential

28–32

95 °C (15 sec), 68–72 °C (70 sec)

  

72 °C (2 min)

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