Table 3 Sequence-specific primers (SSPs) for the VRN1 gene from Poaceae.
ID | Sequence (5′-3′) | Information | Tm (°C) | CG (%) | LC (%) | Lolium perenne, DNA (JN969603) | Lolium perenne, mRNA (AY198326) |
|---|---|---|---|---|---|---|---|
5315 | CTSAAGCGGATCGAGAACAAGATCAACC | F, exon1 | 61.5 | 50.0 | 78 | 185→212 | |
5410 | CTCATCATCTTCTCCACCAAGGGAAAGCTCTACGAGTTC | F, exon1 | 66.2 | 48.7 | 81 | 296→334 | |
5299 | GTTCTCGATCCGCTTSAGCTGCACCTT | R, exon1 | 64.5 | 55.6 | 89 | 176←202 | |
5411 | GCACGGAGATCTCGTGCGCCTTCTTGAG | R, exon1 | 66.8 | 60.7 | 89 | 248←275 | |
5412 | CTCGTAGAGCTTTCCCTTGGTGGAGAAGATGATGAG | R, exon1 | 65.3 | 50.0 | 80 | 296←331 | |
5317 | ARCGGTAYGAGCGYTACTCYTATGCAGA | F, exon2 | 62.3 | 50.0 | 87 | 14265→14292 | 366→393 |
5413 | GARCGGTATGAGCGCTAYTCYTATGCAGA | F, exon2 | 62.4 | 50.0 | 83 | 14264→14292 | 365→393 |
5300 | TARGAGTARCGCTCRTACCGYTCAAGAA | R, exon2 | 60.5 | 46.4 | 83 | 14259←14286 | 360←387 |
5301 | GTARCGCTCRTACCGYTCAAGAATTTTGTCCATA | R, exon2 | 62.8 | 42.6 | 88 | 14248←14281 | 349←382 |
5416 | CAGCCGTTGATGTGGCTCACCATCCA | R, exon8 | 64.7 | 57.7 | 93 | 16421←16441 | 872←897 |
5445 | CTTGTTTTGGGCCGTCTCGCTTC | R, promoter | 61.2 | 56.5 | 73 | promoter primer | |
5446 | CGTCTCGCTTCTCCCGTTTGGGCAT | R, promoter | 64.9 | 60.0 | 81 | ||
