Figure 4

FAM E3 interference with the cell lipid metabolism of the host cells. Vero cells were infected with ZIKV at MOI = 0.1 and treated with FAM E3 3 µM or DMSO 0.1% or OLX controls for 72 h. Naïve Vero cells were treated with DMSO were used as non-infected cells control. After treatment, cells were fixed and nuclei, lipid droplets (LDs) and ZIKV NS3 were labeled using DAPI (blue), BODIPY 493/503 (green) and ZIKV anti-NS3 antibody (red), respectively. Scale bar 100 nm.