Figure 4
Statistical analysis of histopathological scores. (A) *P = 0.021 compared with the model group. Data are expressed as the \(\overline{{\rm{x}}}\) ± SD. IP and IV indicate the UCMSC IP and IV injection groups, respectively. (B) *P = 0.032 compared to the model group. Data are expressed as the \(\overline{{\rm{x}}}\) ± SD. Colon goblet cell staining (100× magnification). (C–F) Are the normal control group, model group, hUCMSC IV group and hUCMSC IP group, respectively. Glycogen granules in goblet cells are stained purple. After hUCMSC treatment of acute IBD model mice, PAS staining of the colon showed a diffuse reduction in or loss of goblet cells in the model group and more goblet cells in the UCMSC treatment group. Colon collagen deposition (100× magnification). In the acute IBD study, colon collagen staining showed that the collagen deposition area or range in the model group was significantly larger than that in the cell treatment group (H), indicating that the distal colonic hardening in mice in the model group is likely due to fibrosis. Collagen is stained dark blue. (G–J) are the normal control group, model group, hUCMSC IV group and hUCMSC IP group. UCMSC tracer. (K–M) Staining for UCMSCs in the mouse colon, spleen, and kidney. The brownish-yellow cell area is indicated by a red arrow (200× magnification). Trace staining of hUCMSCs. (N–P) Trace staining of hUCMSCs in spleen, liver and lung tissues. The brownish-yellow area (black arrow) indicates the presence of hUCMSCs (200× magnification). The staining of the spleen tissue was darker and more widely distributed, indicating the greater distribution of UCMSCs.
