Figure 2
From: A Pre-clinical Animal Model of Secondary Head and Neck Lymphedema
Schematic of the cervical lymphadenectomy model, including identification and removal of cervical lymph nodes using Prox1-EGFP lymphatic reporter rats. (A) To induce cervical lymphedema, rats underwent surgical dissection of the superficial cervical lymph nodes (red ovals) and deep cervical lymph nodes (red diamonds overlying the carotid sheath) followed by irradiation. (B) Superimposed images of superficial cervical lymph nodes located around the submandibular glands and the same lymph nodes under green fluorescence microscopy in a Prox1-EGFP lymphatic reporter rat. (C) Intraoperative image of rat neck identifying the field of view for 2D. (D) Identification of the superficial cervical lymph nodes: mandibular lymph node (MLN) located superiomedial to the submandibular gland, accessory mandibular lymph node (AMLN) located superiolateral to the submandibular gland, superficial parotid lymph node (SPLD) overlying the external jugular vein. (E) Intraoperative image of rat neck identifying the field of view for 2F. (F) Identification of the deep cervical lymph nodes (DCLN) located deep to the junction of the sternohyoid (SH), omohyoid (OH) and sternocleidomastoid (SCM) muscles and overlying the carotid sheath.
