Figure 7
From: A Pre-clinical Animal Model of Secondary Head and Neck Lymphedema
Lymphatic injury results in characteristic fatty deposition, inflammation and fibrosis as seen in human lymphedema. (A) H&E analysis of neck tissue from lymphadenectomy rats reveals a 38% increase in dermis thickness compared to sham rats (P = 0.12) (B) H&E analysis of neck tissue from lymphadenectomy rats reveals an 83% increase in subcutis thickness compared to sham rats (P = 0.008) (C) Quantified IHC sections of CD3-stained tissues reveal a 114% greater CD+ T-lymphocyte density in the lymphadenectomy group compared to controls (P = 0.005). (D) Picrosirius red staining of neck tissue from lymphadenectomy rats reveals a 22% increase in collagen density per total tissue area compared to sham rats (P = 0.001). (E) Quantified IHC sections of TGFβ1-stained tissues reveal a 110% greater TGFβ1 +cell density in the lymphadenectomy group compared to controls (P = 0.04). (F) Quantified reverse transcriptase polymerase chain reaction of rat neck tissue reveals a 1.7-fold change in TGFβ1 mRNA expression in the lymphadenectomy group compared to controls (P = 0.03).
