Figure 6

Effect of MA on apoptosis in PBMCs. Living cells were detected on the basis of a lack of PI fluorescence and a high level of DiOC6(3) fluorescence (PI-DiOC6(3)hi). Cells in the early stages of apoptosis were identified using the criterion of an absence of PI fluorescence with low DiOC6(3) fluorescence (PI-DiOC6(3)-/low). Cells in late apoptosis and/or necrosis were identified using the PI + DiOC6(3)-/low phenotype. Flow cytometry analyses of PI/ DiOC6(3) fluorescence in MA⁻Inf⁻ (A), MA⁺Inf⁻(B), MA⁻Inf⁺ (C), and MA⁺Inf⁺ (D). The DiOC6(3)/PI staining assay was used to quantify the number of apoptotic cells in early apoptosis (E) and late apoptosis (F). In the table below the graphs, the p-value significance levels, obtained by comparing the groups using a single-factor analysis of variance (ANOVA), followed by a pairwise comparison using the Tukey test, are indicated. Differences were considered significant when p-value < 0.05. Data are represented as mean ± SD.