Figure 1
Schema of experimental design for study 1 and 2. The time line of drug treatments, exposures, and necropsies are indicated by arrows. Before air or ozone exposures began, rats were injected with vehicle (Saline, SAL), clenbuterol (CLEN), or dexamethasone (DEX) in study 1 and SAL or CLEN followed by DEX (CLEN + DEX) in study 2 at dose levels indicated. Injections were done in the mornings (~6–7 AM) daily starting one day prior to (D-1) and each day of exposure (D 1 and D 2). For both studies, animals were exposed to air or 0.8 ppm ozone (4 hr/day) for 2 consecutive days. Whole body plethysmography was performed within 30–45 min after each exposure day. Glucose tolerance testing was performed within 0.5–2.5 hr following first day of ozone exposure (D 1) after plethysmography, while necropsy and tissue collection were performed within 0.5–2.5 hr post second day of exposure (D 2) and plethysmography.
