Figure 4 | Scientific Reports

Figure 4

From: Genetic and epigenetic alterations induced by bisphenol A exposure during different periods of spermatogenesis: from spermatozoa to the progeny

Figure 4

Quantification of γH2AX (A) and 53BP1 (B) in F1 embryos from males exposed to BPA during mitotic phase. Boxes represent median of around 200 cell of 5 embryos per treatment (n = 5). Asterisks indicate significant differences (**p < 0.01; ***p < 0.001) when comparing to control group. Representative images of 3.3hpf-embryo at 5X (scale bar = 50 µm) appeared in (C). Channels were both separated in blue (DAPI), red (Alexa Fluor 568) and green (Alexa Fluor 488) and merged. The graphic represents the co-localization of red channel (γH2AX) in X–axis and green channel (53BP1) in Y-axis.

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