Table 4 TH staining of the striatum in the brain by IHC was performed on these 21 NGR rats which were fed semipurified hiCHO diets (60:20:20) with or without 10% PFB for 8 weeks, which were subdivided into diabetes-susceptible or diabetes-resistant rats based on an RBG ≥ 75 mg/dl.

From: Palm Fruit Bioactives augment expression of Tyrosine Hydroxylase in the Nile Grass Rat basal ganglia and alter the colonic microbiome

PFB status

Diabetes status

n

Mean optical density*

No PFB

Diabetes resistant

5

0.14 ± 0.04a

Diabetes susceptible

5

0.16 ± 0.03b

Severe diabetes

3

0.15 ± 0.06

+PFB 10%

Diabetes resistant

4

0.42 ± 0.09a

Diabetes susceptible

4

0.38 ± 0.07b

  1. a,bSignificant difference (p < 0.05),  by two-way ANOVA.
  2. *Mean optical density is proportional to the concentration of the stain. Optical density = mean ± SEM.
  3. Image mean density collected by Nikon software from area ~5000 nm2 from subsection of the positively stained TH area (striatum).
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