Figure 4 | Scientific Reports

Figure 4

From: Identification of Acinetobacter baumannii and its carbapenem-resistant gene blaOXA-23-like by multiple cross displacement amplification combined with lateral flow biosensor

Figure 4

Detection of multiplex targets in a m-MCDA reaction. Two sets of MCDA primers targeting pgaD- and blaOXA-23-like-MCDA genes were simultaneously added to a reaction tube and the LoD of m-MCDA for simultaneously detecting pgaD and blaOXA-23-like genes was confirmed using LFB. Biosensors 1, 2, 3, 4, 5 and 6 represent DNA levels of 10 ng (A. baumannii SG-AB001), 10 pg (A. baumannii SG-AB001), 1 pg (A. baumannii SG-AB001), 100 fg (A. baumannii SG-AB001), 10 fg (A. baumannii SG-AB001) and 1 fg (A. baumannii SG-AB001); biosensor 7, negative control (K. pneumoniae); biosensor 8, blank control (DW). The LoD of m-MCDA assay for pgaD and blaOXA-23-like detection was 100 fg per vessel.

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