Figure 6

Detection of glial cells in the brain tissue from BALB/c mice infected with DENV2. Tissue samples were collected from animals inoculated with mock (A,B,E,F) or with DENV2 (10 dpi) (C,D,G,H). Sections were incubated with anti-GFAP (A–D) or anti-IBA-1 (E–H) antibody, followed by incubation with peroxidase-conjugated rabbit anti-IgG antibody. Detection of astrocytes by the GFAP (A–D) and microglia by the IBA-1 protein (E–H). The sections were stained with Harris hematoxylin. Mi – microglia; as – Astrocyte. Bars (B,D,F,H) = 10 μm; (A,C,E) = 25 μm; (G) = 50 μm.