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Figure 1

From: Phosphoproteomic analysis sheds light on intracellular signaling cascades triggered by Formyl-Peptide Receptor 2

Figure 1

Phosphoproteomic analysis of WKYMVm-stimulated CaLu-6 cells. (a) Schematic diagram of the experimental workflow applied for the analysis of CaLu-6 phosphoproteome in response to WKYMVm stimulation by nano-liquid chromatography coupled with high-resolution tandem mass spectrometry. Total proteins were purified in control (CTR) and stimulated (TRT) cells. Phosphoproteins were enriched by TiO2 procedure, concentrated by ultrafiltration and digested with tripsin. (b) Pie chart of the phosphorylated amino acid distribution.

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