Figure 7
LXA4 induces HSP-27, OSR1, Rb and MARCKS phosphorylation. CaLu-6 cells were serum-starved for 24 hours and stimulated or not with 1 μM LXA4 for 5 minutes. Cells were also preincubated with 10 μM WRW4 or 100 ng/mL PTX before LXA4 stimulation. Whole lysates (50 μg) were resolved on 10% SDS-PAGE and hybridized with anti-p-HSP-27(Ser82) (a); anti-p-OSR1(Ser339) (b); anti-p-Rb(Ser608) (c); anti-p-MARCKS(Ser170) (d), and anti-p-MCM2(Ser139) antibodies. Proteins were detected by autoradiography and bands were estimated from densitometric scanning. An anti-tubulin antibody was used to normalize the amount of loaded proteins. Results are representative of three independent experiments performed in triplicate.
