Table 1 Effect of NAA at different concentrations on induction of somatic embryos from root, stem and leaf explants of R. sceleratus.

From: NAA at a high concentration promotes efficient plant regeneration via direct somatic embryogenesis and SE-mediated transformation system in Ranunculus sceleratus

NAA (mg/L)

Number of somatic embryos induced per explant

Root

Stem

Leaf

0

0.00 ± 0.00 kJ

0.00 ± 0.00 kJ

0.00 ± 0.00 kJ

1.0

3.90 ± 0.44 jkIJ

6.57 ± 0.42 ijHI

9.33 ± 0.49 iH

2.5

14.80 ± 0.44 hG

20.03 ± 0.73 gF

19.37 ± 0.74 gFG

5.0

22.60 ± 0.55 gEF

27.47 ± 0.62 fE

35.17 ± 0.68 eD

10.0

66.03 ± 0.80 cC

126.47 ± 2.23 bB

213.63 ± 4.76 aA

20.0

27.33 ± 0.85 fE

35.13 ± 0.60 eD

39.23 ± 0.50 dD

  1. The mean and standard error per treatment were calculated from 300 explants on 30 petri dishes (treated as 30 replicates). Capital and lowercase letters indicate a significant difference at the 1% and 5% probability level respectively. Significant differences were analyzed with a Duncan test using SPSS 16.0.
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