Figure 6 | Scientific Reports

Figure 6

From: Unpacking the aggregation-oligomerization-fibrillization process of naturally-occurring hIAPP amyloid oligomers isolated directly from sera of children with obesity or diabetes mellitus

Figure 6The alternative text for this image may have been generated using AI.

(A) Results from the ThT fluorescence experiments. (A) Group A-T1DM, (B) Group B-T2DM, (C) Group C-Obesity, (D) Group D-Healthy children. Representative sample from every group was analyzed at two different concentrations, and in absence of synthetic monomeric hIAPP. A blank sample was included for comparison. All measurements were performed with a ThT-buffer at 37 °C (PBS 1X, pH 7.4 + 20 μM ThT); protein concentrations of 0.039 mg/mL or 0.0039 mg/mL (B) Comparison of the average t-lag for the trapping experiment (three measurements). One sample from every group was added to synthetic monomeric hIAPP at the same concentration 1:1 (0.039 mg/mL each one), and at one tenth of the concentration 1:0.1 (0.039 mg/mL synthetic hIAPP: 0.0039 mg/mL patient PTS). Synthetic Monomeric hIAPP in absence of patient PTS is included as reference.

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