Figure 4
From: Fluorescence imaging reversion using spatially variant deconvolution
SAIRC improvement in quality of fluorescence images of mice bearing HCT116 tumors expressing IRFP720. The upper row corresponds to one mouse, while the lower row corresponds to a second mouse. (a,e) Color images of the abdominal area, after embedding in OCT compound. Scalebar: 1 mm. (b,f) Raw fluorescence image of the tissue block in panels (a) and (e). (c,g) Corrected image after the implementation of SAIRC (d,h). (i) Fluorescence image of a thin slice (50 μm) of the tissue block obtained under the same conditions as the block image. (ii) Stitched images of the thin slices obtained from a commercial fluorescence microscope. White arrows indicate tumor features that become clearly distinguishable only after SAIRC correction.
