Figure 3
Effect of LPSEC on platelet activation. The level of fibrinogen binding (as a marker for inside-out signalling to integrin αIIbβ3) (A) and the level of P-selectin exposure (as a marker for α-granule secretion) (B) was measured in PRP upon incubation with LPSEC for 20 minutes at room temperature (n = 4). In order to determine the impact of temperature on LPS-induced effects in platelets, the level of fibrinogen binding (C) and P-selectin exposure (D) in PRP was measured by incubating PRP with LPSEC for 25 minutes at 37 °C (n = 5). To determine if the LPS chemotypes possess priming effects in platelets, the level of fibrinogen binding (E) and P-selectin exposure (F) was measured in PRP upon preincubation with LPSEC for 5 minutes followed by stimulation with a vehicle control or 0.5 μg/mL CRP-XL for 20 minutes at 37 °C (n = 3). Similarly, the level of fibrinogen binding (G) and P-selectin exposure (H) in human whole blood was measured upon incubation with LPSEC for 25 minutes at 37 °C (n = 3). The data were normalised to either their resting control (100%: A–D and G,H) or their 0.5 μg/mL CRP-XL control (100%; E,F) and analysed using one-way ANOVA and Dunnett’s post-hoc test. Data represent mean ± S.D. 2 EC - 2 μg/mL LPSEC.
