Figure 4
Effect of LPSSM on platelet activation. To determine the impact of LPSSM on the modulation of platelet activation, the level of fibrinogen binding (A) and P-selectin exposure (B) was analysed in PRP in the presence of different concentrations of LPSSM and 0.5 μg/mL CRP-XL after simultaneous incubation for 20 minutes at room temperature (n = 5). To determine the impact of temperature on LPS-mediated effects in platelets, the level of fibrinogen binding (C) and P-selectin exposure (D) in PRP was measured upon preincubation with LPSSM for 5 minutes followed by stimulation with 0.5 μg/mL CRP-XL for 20 minutes at 37 °C (n = 6). Similarly, the preincubation of PRP with LPSSM was increased to 30 minutes prior to stimulation with 0.5 μg/mL CRP-XL for 20 minutes at 37 °C, and the level of fibrinogen binding (E) and P-selectin exposure (F) was analysed (n = 4). Furthermore, the level of fibrinogen binding (G) and P-selectin exposure (H) was analysed in human whole blood upon preincubation with LPSSM for 5 minutes followed by stimulation with 0.5 μg/mL CRP-XL for 20 minutes at 37 °C (n = 3). The data were normalised to their 0.5 μg/mL CRP-XL control, and the p value (**p < 0.01) shown was calculated using one-way ANOVA and Dunnett’s post-hoc test. Data represent mean ± S.D. The samples treated in the absence of 0.5 μg/mL CRP-XL are represented with empty bars.
