Figure 1
From: DPP8 is a novel therapeutic target for multiple myeloma
Cytotoxic effects of DPP4 inhibitors against multiple myeloma cell lines. (A) 1.0 × 105 MM.1 S (open circles) or RPMI8226 (closed circles) cells were cultured at doses of 0–100 µM DPP4 inhibitors (vildagliptin, saxagliptin, sitagliptin, alogliptin, or linagliptin) for 72 hours. Cell number was estimated by a colorimetric assay using WST-1 reagent (n = 6). (B) 1.0 × 105 MM.1 S cells were cultured with 100 µM DPP4 inhibitors (vildagliptin, saxagliptin, sitagliptin, alogliptin, or linagliptin) for 72 hours. Cell number was estimated by a colorimetric assay using WST-1 reagent (n = 6). (C) 1.0 × 105 Karpas 299 cells were cultured with 100 µM DPP4 inhibitors (vildagliptin, saxagliptin, sitagliptin, alogliptin, or linagliptin) for 24 hours, respectively. DPP4 activity was estimated using a luminogenic DPP4 substrate, Gly-Pro-aminoluciferin (n = 6). The data are representative of three separate experiments and presented as the mean ± SD.
