Figure 2
Maternal separation (MS) pups produce more high frequency calls and adjust low: high call frequency ratio in response to disrupted maternal reunion. (A) In the first isolation (ISO1), MS pups’ high frequency call proportion had a mean±SEM of 0.43 ± 0.04 (dark red) compared to 0.36 ± 0.06 in control (dark blue). (B) In the second isolation (ISO2), MS pups’ high frequency call proportion increased to 0.51 ± 0.04 (dark red) whereas control pups’ high frequency call proportion stayed about the same, 0.34 ± 0.04 (dark blue) (2-way repeated measure ANOVA; group: F(1,26) = 4.09, p = 0.054; isolation: F(1,26) = 1.64, p = 0.21, group x isolation interaction: F(1,26) = 3.69, p = 0.066).
