Figure 4

Call number by isolation and change score. In the first isolation (ISO1) the call number emitted by maternal separation (MS) pups was similar to control pups (mean ± SEM); MS: 467 ± 62 USVs/3 min, control: 457 ± 60 USVs/3 min. In the second isolation (ISO2) both groups emitted more calls; MS: 962 ± 130 USVs/3 min, control: 686 ± 60 USVs/3 min. Isolation period, but not group, had a significant effect on calling rate (2-way ANOVA with repeated measures- group: F_(1,26) = 1.52, p = 0.23, isolation: F_(1,26) = 29.79, p < 0.0001, group x isolation interaction: F_(1.26) = 3.77, p = 0.063). Furthermore, disrupting the reunion potentiated calls in both groups (Post hoc t-test with Bonferroni correction; MS: t(26) = 5.23, p < 0.0001, d = 1.26, control: t(26) = 2.49, p = 0.039, d = 0.81. *indicates a comparison between ISO1 where p < 0.05, ***p < 0.001indicates a comparison to ISO1 where p < 0.0005.