Figure 1 | Scientific Reports

Figure 1

From: Sodium valproate and 5-aza-2′-deoxycytidine differentially modulate DNA demethylation in G1 phase-arrested and proliferative HeLa cells

Figure 1

5mC global levels in synchronized and non-synchronized HeLa cells analysed by flow cytometry (a,b), ELISA (d) and confocal microscopy (c,e). Non-synchronized cells were analysed for 5mC content according to their cell cycle phases. 5mC/DNA data were calculated from the mean fluorescence intensity (mfi) of 5mC vs. propidium iodide (PI) (a). The 5mC content was also analysed in synchronized cells collected in the G1 (b–d) and S (b,d,e) phases. Using Student’s t-test, significant differences at P < 0.05 between the treatments and respective controls are indicated (*). A significant decrease in 5mC in VPA- treated, G1-arrested cells under non-proliferative (b–d), proliferative (b,d,e) and non-synchronized (a) cells was observed for all assays. After treatment with 5-aza-CdR, this decrease was observed only in cells collected or selected from the S phase (a,b,d,e). Data are representative of three independent experiments for flow cytometry and ELISA and two independent experiments for confocal microscopy. Scale bars indicate 20 μm.

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