Figure 3 | Scientific Reports

Figure 3

From: Sodium valproate and 5-aza-2′-deoxycytidine differentially modulate DNA demethylation in G1 phase-arrested and proliferative HeLa cells

Figure 3

Gene expression of DNMT1, TET1 and TET2 in VPA- and 5-aza-CdR- treated, synchronized HeLa cells. VPA induced decreased DNMT1 gene expression levels in cells at the G1 and S phases, whereas 5-aza-CdR only promoted this decrease in cells in the S phase (a,b). The TET2 gene expression was altered after treatments with 1 and 20 mM VPA in both phases of the cell cycle but remained unaltered in response to 5-aza-CdR (c,d). TET1 gene expression levels increased after VPA treatment in the G1-arrested cells (e). Data obtained from RT-PCR analyses were normalized to endogenous GAPDH controls. Using Student’s t-test, significant differences at P < 0.05 between the treatments and respective controls are indicated (*). Data are representative of three independent experiments.

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