Figure 4

DNMT1, TET2 and TDG protein levels in VPA- and 5-aza-CdR-treated, synchronized HeLa cells. Western blots and densitometry quantifications of DNMT1, TET2 and TDG are shown. The DNMT1 levels increased compared to the respective controls after treatment with 20 mM VPA in proliferating cells (b) but not in G1-arrested cells (a). The TET2 protein levels were significantly affected by treatment with 1 mM and 20 mM VPA compared to the respective controls in G1-arrested (c) and proliferating cells (d) and by treatment with 5-aza-CdR in proliferating cells (d). The TDG protein levels decreased in replicating cells (f) but not in G1-arrested cells (e) after cell exposure to 20 mM VPA and 5-aza-CdR. GAPDH was used as a loading control. Using Student’s t-test, significant differences at P < 0.05 between treatments and respective controls are indicated (*). Three to seven independent experiments were performed, and blots were processed in parallel. The grouping of blots including the loading control and specific proteins, was derived from the same experiment. Full-length blots are included in the Supplementary Information file [Supplementary Fig. S5].