Figure 4 | Scientific Reports

Figure 4

From: Overexpression of the HECT ubiquitin ligase PfUT prolongs the intraerythrocytic cycle and reduces invasion efficiency of Plasmodium falciparum

Figure 4

Colocalization of PfUT-HA with ER and Golgi markers in pfut mutants. Indirect immunofluorescence assay (IFA), using pfut mutants (at the trophozoite stage) and an anti-HA antiserum (mouse, 1:1000) together with the ER marker BiP (rabbit, 1:1000) or ERC (rabbit, 1:500), or with the Golgi marker ERD2 (rabbit, 1:500). Secondary antibodies were an anti-mouse Alexa Fluor 488 (green) and an anti-rabbit Alexa Fluor 546 (red). The nuclei were visualized with Hoechst (blue). Different channels, the differential interference contrast (DIC) image and two overlay images are shown for two representative examples each. The plot profiles are included for a better presentation of signal colocalization. Colocalization of anti-HA and anti-PfUT fluorescence signals is shown in Supplementary Fig. 7. Scale bar: 5 µm. This figure was reproduced from the PhD thesis by Jankowska-Döllken32.

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