Figure 5

Subcellular localization of PfUT by immunoelectron microscopy. (a) A representative micrograph is shown of an erythrocyte infected with a pfut mutant. The sample was prepared according to the Tokuyasu protocol, and immunolabelled with anti-HA antiserum (mouse, 1:1), followed by staining with an anti-mouse antibody coupled to 10 nm colloidal gold (goat, 1:20). Arrowheads point towards gold label. n, nucleus; ER, endoplasmic reticulum/Golgi complex. Scale bar, 200 nm. (b) Quantification of immuno EM results. The distribution of gold grains was determined in 5 micrographs and analyzed according to their subcellular localization. Gold grains were significantly more present in areas of the ER/Golgi complex (ER) than in other subcellular compartments, including the parasite’s cytoplasm (C), nucleus (N), digestive vacuole (DV), red blood cell cytosol (RBC) and non-cellular background (BG). Each symbol represents data derived from an individual parasitized erythrocyte. A box plot analysis is overlaid over the individual data points, with the median (thin grey line), mean (thick black line) and the 25% and 75% quartile ranges being shown. Statistical significance between the data sets was assessed using the Holm-Sidak one way ANOVA test. This figure was reproduced from the PhD thesis by Jankowska-Döllken³².