Figure 7

Analysis of the cell cycle length in pfut mutants and 3D7. The DNA copy number (C-value) was determined in highly synchronized cells in intervals of 4 h throughout the 48 h replicative cycle. (a) Cell cycle in the absence of GlcN; (b) after one cycle in the presence of 5 mM GlcN; (c) after three cycles in the presence of 5 mM GlcN. The drop in the C-value after reaching the peak indicates the beginning of a new cycle. Each data point represents the mean ± SD of three independent biological replicates. The G1 and S/M-like phase of the 48 h intraerythrocytic cell cycle of 3D7 are indicated to better display the differences with pfut mutants. (d) Cell cycle length as a function of GlcN treatment for 48 h (one replicative cycle) and 144 h (three replicative cycles) in pfut mutants and 3D7. ^*p < 0.001, compared with the untreated pfut mutant cell lines, according to Student’s t-test. This figure was reproduced from the PhD thesis by Jankowska-Döllken³².