Table 1 Cell cycle characteristics for pfut mutants and 3D7.

From: Overexpression of the HECT ubiquitin ligase PfUT prolongs the intraerythrocytic cycle and reduces invasion efficiency of Plasmodium falciparum

Treatment

Clone

Onset of DNA replication

[h p.i.]

Peak of the C-value

[h p.i.]

Length of the S/M-like

phase [h]

1st cycle

(−GlcN)

3D7

26 ± 2

46 ± 2

20 ± 2

5 G

26 ± 2

54 ± 2*

28 ± 2*

6E

26 ± 2

54 ± 2*

28 ± 2*

11B

26 ± 2

54 ± 2*

28 ± 2*

1st cycle

(+GlcN)

3D7

26 ± 2

46 ± 2

20 ± 2

5 G

26 ± 2

54 ± 2*

28 ± 2*

6E

26 ± 2

54 ± 2*

28 ± 2*

11B

26 ± 2

54 ± 2*

28 ± 2*

3rd cycle

(+GlcN)

3D7

28 ± 2

46 ± 2

18 ± 2

5 G

28 ± 2

50 ± 2*,#

22 ± 2*,#

6E

28 ± 2

50 ± 2*,#

22 ± 2*,#

11B

28 ± 2

50 ± 2*,#

22 ± 2*,#

  1. The following parameters are indicated: the onset of DNA replication, as defined by the time point post invasion (p.i.) when the C-value reaches 2; the time point of maximal C-value; and the length of the S/M phase. Data are shown for cells cultured in the absence of GlcN and cells treated for one and three replicative cycles with 5 mM GlcN. *p < 0.001, compared with the parental line 3D7, according to Holm-Sidak one way ANOVA. #p < 0.001, compared with untreated pfut mutant cultures, according to Holm-Sidak one way ANOVA.
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