Figure 2 | Scientific Reports

Figure 2

From: β-catenin activation down-regulates cell-cell junction-related genes and induces epithelial-to-mesenchymal transition in colorectal cancers

Figure 2

Cell cycle and cell-cell adhesion-related pathways were up- and down-regulated, respectively, in HCT116-MT cells, compared to HCT116-WT cells. (a) Transcriptional profiles of HCT116-WT and HCT116-MT cells were measured by Illumina HumanHT-12 v4 Expression BeadChip microarray. Gene ontology (GO) analysis was then performed on the list of 1,507 differentially expressed genes (DEGs) showing >1.5-fold up- or down-regulation in HCT116-MT vs. HCT116-WT cells with P < 0.05, using Cytoscape method to identify pathways enriched or suppressed in β-catenin mutant cells. (b) A curated gene list was obtained by overlapping the list DEGs identified by microarray analysis with a set of 128 cell cycle-related genes and a set of 134 cell adhesion-related genes obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG). Fold change (F.C.) in HCT116-MT relative to HCT116-WT cells is shown. (c) qRT-PCR validation of the microarray results was performed using six randomly selected genes. Error bars represent the SD of the mean of two independent experiments.

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