Figure 3
From: Myc stimulates cell cycle progression through the activation of Cdk1 and phosphorylation of p27
Myc-mediated degradation of p27. (a) Protein levels of Myc, Skp2, Cdk1 and p27 of Cdk2−/− MER MEFs grown until confluence and treated with 500 nM of 4HT for 18 hours (overnight). Actin levels were used as loading control. (b) Protein stability of p27 in Cdk2−/− Lv141 and Cdk2−/− Myc MEFs transfected with a p27-YFP construct measured by western blot. Levels of p27-YFP were detected after 0, 0.5, 1 and 3 hours of cycloheximide treatment (30 µg/mL). Actin levels were used as loading control. (c) Densitometric quantification of p27 protein levels after cycloheximide treatment normalized to actin levels. Error bars represent ± SD of three independent experiments. Statistical analysis: T.test: *p < 0.1; **p < 0.05.
