Table 1 Mass spectrometric analysis of tryptic peptides deprived from the Mono S Fr. 1 and L-chain fraction of 64M-5 Fab.
Residue No.a | Sequences | Calculated mass | Observed mass | Peak No.b |
|---|---|---|---|---|
1–24 | DVLMTQTPLSLPVSLGDQASISCR | 2,591 | 2,583 | 27 |
25–45 | SSQNIVHSNGYTYLEWYLQKPGQSPK | 3,026 | 3,029 | 25 |
46–54 | LLIYTVSNR | 1,079 | 1,080 | 11 |
55–74 | FSGVPDRFSGSGSGTDFTLK | 2,063 | 2,061 | 22, 23 |
75–90 | ISRVEAEDLGVYYCFR | 1,979 | 1,980 | 15 |
91–103 | GSHVPTFGGGTK | 1,145 | 1,147 | 12 |
104–107 | LEIK | 503 | — | — |
108–142 | RADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPK | 3,731 | 3,735 | 31 |
143–147 | DINVK | 589 | — | — |
148–155 | WKIDGSER | 991 | 992 | 13 |
156–169 | QNGVLNSWTDQDSK | 1,593 | 1,597 | 17, 18 |
170–188 | DSTYSMSSTLTLTKDEYER | 2,228 | 2,229 | 21 |
189–199 | HNSYTCEATHK | 1,349 | 1,353 | 6 |
200–207 | TSTSPIVK | 833 | 835 | 8 |
208–211 | SFNR | 524 | 524 | 5 |
212–214 | NEC | 423 | — | — |
