Figure 2 | Scientific Reports

Figure 2

From: The small molecule inhibitor anle145c thermodynamically traps human islet amyloid peptide in the form of non-cytotoxic oligomers

Figure 2

Effect of hIAPP and anle145c on INS-1E and MJS cells. Top: Microscopy images of INS-1E cells 6 h after incubation showing (A) untreated cells, (B) cells incubated with 10 µM hIAPP, and (C) cells incubated with 1 µM anle145c and 10 µM of hIAPP. Middle: Microscopy images of MJS cells 24 h after incubation showing (D) untreated cells, (E) cells incubated with 10 µM hIAPP, and (F) cells incubated with 1 µM anle145c and 10 µM of hIAPP. The scale bars represent 175 µm. Bottom: Quantification of the percentage of dead cells after 24 h of incubation, revealed by DAPI-staining for INS-1E cells (G) and MJS cells (H). Both panels show from left to right untreated and DMSO treated cells, followed by 10 µM hIAPP in the presence of different concentrations of anle145c, and 5 µM anle145c in the absence of hIAPP as indicated. All samples, except for the untreated cells, contained the same final DMSO concentration (5 vol %). The error bars represent the standard error from two independent experiments.

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