Figure 4 | Scientific Reports

Figure 4

From: Adeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly

Figure 4

Impact of AAP2 on in vitro assembly. (a) Schematic representation of AAP2 expression construct. A His-tag coding sequence was added 3′of the AAP2 gene. (b) Coomassie SDS-PAGE; (1) protein standard; (2) insoluble fraction of intracellular protein before purification; (3) IMAC purified protein; (4) Western blot of the insoluble fraction detected with anti-His-tag antibody. (c) ELISA after in vitro assembly using the same final protein concentration (50 µg/ml) with different ratios of VP3wt:AAP2. Full-length gel and blot are presented in Supplementary Fig. S4.

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