Table 3 Canonical gene set enrichment analysis (GSEA) of transcripts that were significantly differentially expressed transcripts in the gut tissues of either male or female C. rosa s.s and C. quilicii. Enrichment profiles established using the WEB-based GEne SeT AnaLysis Toolkit (WebGestalt)⁴⁶. Non-redundant enriched Gene Ontology (GO) categories.

Ceratitis quilicii Male

Biological process

GO:0019730

Antimicrobial humoral response

106

5

0.64

7.81

0.0004

4.93E-02

GO:0030968

Endoplasmic reticulum unfolded protein response

8

2

0.05

41.41

0.001

4.93E-02

GO:0015149

Hexose transmembrane transporter activity

18

2

0.11

18.1

0.0053

1.44E-01

GO:0050777

Negative regulation of immune response

11

2

0.07

30.11

0.0019

4.93E-02

GO:0044724

Single-organism carbohydrate catabolic process

39

3

0.24

12.74

0.0016

4.93E-02

Molecular function

GO:0042803

Protein homodimerization activity

72

3

0.44

6.79

0.0097

1.44E-01

Cellular component

GO:0030662

Coated vesicle membrane

23

2

0.15

13.67

0.0092

6.58E-02

GO:0009897

External side of plasma membrane

8

2

0.05

39.3

0.0011

2.69E-02

GO:0005811

Lipid particle

249

7

1.58

4.42

0.0009

2.69E-02

GO:0045298

Tubulin complex

10

2

0.06

31.44

0.0017

2.69E-02

GO:0030018

Z disc

21

2

0.13

14.97

0.0077

6.58E-02

Ceratitis rosa Male

Biological process

GO:0006164

Purine nucleotide biosynthetic process

72

2

0.11

17.48

0.0056

2.37E-01

Molecular function

GO:0000166

Nucleotide binding

1225

3

1.91

1.57

0.2973

5.79E-01

GO:0045735

Nutrient reservoir activity

5

2

0.01

256.29

2.27E-05

5.00E-04

GO:0016491

Oxidoreductase activity

630

2

0.98

2.03

0.2577

5.79E-01

GO:0022891

Substrate-specific transmembrane transporter activity

649

2

1.01

1.97

0.269

5.79E-01

GO:0008270

Zinc ion binding

875

2

1.37

1.46

0.4023

5.79E-01

Cellular component

GO:0005616

Larval serum protein complex

5

3

0.01

437.29

1.92E-08

3.84E-07

GO:0005811

Lipid particle

249

3

0.34

8.78

0.0041

1.64E-02

GO:0005886

Plasma membrane

603

2

0.83

2.42

0.1983

4.67E-01

Ceratitis quilicii Female

Biological process

GO:0045454

Cell redox homeostasis

50

2

0.23

8.78

0.0216

4.67E-01

GO:0008340

Determination of adult lifespan

128

3

0.58

5.15

0.0203

4.67E-01

GO:0008593

Regulation of Notch signalling pathway

62

2

0.28

7.08

0.0322

5.51E-01

GO:0001666

Response to hypoxia

45

2

0.2

9.76

0.0177

4.49E-01

GO:0007296

Vitellogenesis

8

2

0.04

54.89

0.0006

1.12E-01

Molecular function

GO:0015036

Disulfide oxidoreductase activity

30

2

0.13

14.9

0.0079

7.24E-02

GO:0009055

Electron carrier activity

145

3

0.65

4.62

0.0267

1.47E-01

GO:0051287

NAD binding

39

2

0.17

11.46

0.013

1.02E-01

GO:0050661

NADP binding

12

2

0.05

37.25

0.0013

7.15E-02

GO:0016651

Oxidoreductase activity, acting on NADH or NADPH

51

2

0.23

8.77

0.0217

1.33E-01

GO:0005198

Structural molecule activity

487

7

2.18

3.21

0.0054

7.24E-02

Cellular component

GO:0005576

Extracellular region

814

7

3.86

1.81

0.0847

4.49E-01

GO:0015934

Large ribosomal subunit

102

2

0.48

4.14

0.0839

4.49E-01

GO:0005811

Lipid particle

249

7

1.18

5.93

0.0001

5.30E-03

GO:0005875

Microtubule associated complex

362

5

1.72

2.91

0.0269

4.49E-01

GO:0005700

Polytene chromosome

121

3

0.57

5.23

0.0193

4.49E-01

Ceratitis rosa Female

Biological process

GO:030154

Cell differentiation

1799

2

0.76

2.62

0.1664

3.78E-01

Molecular function

GO:0045735

Nutrient reservoir activity

5

2

0

961.1

1.30E-06

2.60E-06

Cellular component

GO:0005616

Larval serum protein complex

5

3

0

962.04

1.16E-09

1.62E-08

GO:0005811

Lipid particle

249

3

0.16

19.32

0.0003

2.10E-03