Figure 1
Marizomib is cytotoxic to HLRCC cells in vitro and in vivo. (A) UOK262 and UOK262WT cells were treated for 48 hours with either bortezomib (Bort) or marizomib (Mar). Cell viability was assessed by CellTiterGlo; (B) ATP content of UOK262 was evaluated 4 hours post-treatment with marizomib (Mar) using ATPLite assay; (C,D) UOK262 cells were treated with bortezomib, marizomib and carfilzomib. ROS levels were measured after 18 hours treatment using DCF dye (C) and viability was assessed in parallel using CellTiterGlo. The antioxidant NAC was used to counteract the build-up of ROS by pretreating the cells with 5 mM of NAC 4 hours prior to the addition of the proteasome inhibitors; (E) Tumor growth curve of a xenograft study with UOK262 injected in the flank of female nude mice and treated with marizomib or vehicle (see methods). Tumors were measured weekly using calipers and tumor volume was calculated as length × width2. *p < 0.05; Control: DMSO control.
