Figure 5 | Scientific Reports

Figure 5

From: An Optimised Protocol Harnessing Laser Capture Microdissection for Transcriptomic Analysis on Matched Primary and Metastatic Colorectal Tumours

Figure 5

Validation of RNA-Seq data by qPCR analysis of LCM samples. (a) Heat map illustrating the epithelial and stromal transcriptomic landscape of all the RNA-Seq samples across patients. Each row is normalized using Z-score. (b) Results illustrating the expression patterns of epithelial targets (ERBB2 and S100A11) as well as stromal targets (IGFBP7, TIMP1, SPARC & COLIA1) across samples and tissue types. These targets were upregulated specifically in the respective compartments. The data was normalised to β-Actin and analysed using one-way ANOVA. Results are the mean of three biological replicates and standard deviations are shown. (c) Representative immunohistochemical (IHC) staining of IGFBP7, illustrating the scores 1 to 3 for epithelial and stromal staining intensity scoring in our Krukenberg samples. (d) Distribution of intensity scoring for epithelial and stromal staining in Krukenberg samples (p = 0.0961). *p < 0.05, **p < 0.01, ***p < 0.001.

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